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| Oliva,Maria Luiza V.; Sampaio,Misako U.. |
| Obtained from leguminous seeds, various plant proteins inhibit animal proteinases, including human, and can be considered for the development of compounds with biological activity. Inhibitors from the Bowman-Birk and plant Kunitz-type family have been characterized by proteinase specificity, primary structure and reactive site. Our group mostly studies the genus Bauhinia, mainly the species bauhinioides, rufa, ungulata and variegata. In some species, more than one inhibitor was characterized, exhibiting different properties. Although proteins from this group share high structural similarity, they present differences in proteinase inhibition, explored in studies using diverse biological models. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bowman-Birk; Chymotrypsin; Kunitz inhibitors; Plasma kallikrein; Primary structure; Trypsin. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300023 |
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| Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo. |
| ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322 |
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| Bae,Seong Kyeong; Lee,Hyunkyoung; Heo,Yunwi; Pyo,Min Jung; Choudhary,Indu; Han,Chang Hoon; Yoon,Won Duk; Kang,Changkeun; Kim,Euikyung. |
| Abstract Background: Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a serine protease component of Nemopilema nomurai jellyfish venom (NnV) and unveil its characteristics. Methods: To determine the relationship between fibrinolytic activity of NnV and the serine protease, fibrin zymography was performed using metalloprotease and serine protease inhibitors. The biochemical characterization of serine proteases of NnV were determined by the amidolytic assay. Fractions with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nemopilema nomurai; Jellyfish venom; Chymotrypsin; Serine protease; Fibrinolytic activity. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100323 |
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| Guyonnet, Vincent; Johnson, Joyce K.; Long, Peter L.. |
| The role of pancreatic proteolytic enzymes in the excystation process of Eimeria tenella oocysts and sporocysts was studied in vitro. Intact sporulated oocysts were preincubated in phosphate buffer, NaCl 0.9% (PBS) added with 0.5% chicken bile extract in a 5% C02 atmosphere for 30 minutes prior to exposure to either 0.25% (w/v) chicken trypsin, chymotrypsin, pancreatic elastase, or a 1% (w/v) crude extract of unsporulated and sporulated oocysts of E. tenella (Expt.1). No excystation was observed under these conditions. Sporocysts were also incubated under the same conditions without pretreatment in C02. Excystation was observed for sporocysts incubated with either trypsin, chymotrypsin or pancreatic elastase, the best percentage of excystation being... |
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Palavras-chave: Chymotrypsin; Eimeria tenella; Elastase; Excystation; Trypsin. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/155 |
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| Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J. |
| Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases. |
| Tipo: Text |
Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus. |
| Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf |
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